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anti huiga apc mab  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti huiga apc mab
    Anti Huiga Apc Mab, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iga+apc/IgA+Antibody%2C+anti-human/pmc13387492-32-0-3
    Average 96 stars, based on 352 article reviews
    anti huiga apc mab - by Bioz Stars, 2026-10
    96/100 stars

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    Staining:

    Article Title: An efficient approach to study ANA⁺ B cells in autoimmune diseases integrating flow cytometry with single-cell analysis.
    Article Snippet: .. Conjugated antibody cocktails were prepared in 300 μL staining buffer containing CD19PECy7 (Cat. no. 302216, BioLegend, San Diego, CA, USA), CD27-PE (Cat. no. 50-103-00, eBioscience, Fisher Scientific), CD38-PE-Texas Red (Cat. no. 61-0389-42, Fisher Scientific), IgA-APC (Cat. no. 130-113-472, Miltenyi Biotec, Bergisch Gladbach, Germany), Streptavidin-APC (Cat. no. 405207, BioLegend), CD1c-BV421 (Cat. no. 331526, BioLegend), IgG-FITC (Cat. no. 555786, BD Biosciences, San Jose, CA, USA), IgM-BV421 (Cat. no. 562380, BD Biosciences), and Fixable Viability Dye eFluor506 (Cat. no. 65-0866- 14, Fisher Scientific). ..

    Article Title: Interferon activation in bone marrow long-lived plasma cells in systemic lupus erythematosus
    Article Snippet: Fixed cells were incubated with Fc block solution (Human TruStain FCx, BioLegend, #422302), surfaced stained with antibodies for 30 minutes at 4°C followed by LIVE/DEADTM Fixable Aqua Dead Cell Stain Kit (Invitrogen, L34966). .. Surface stain antibodies include CD3-ALX700 (clone SP34-2, BD 557917), CD11c- BUV395 (clone B-ly6, BD563787), CD14-ALX700 (clone M5E2, BD 557923), CD19-BV605 (clone SJ25C1, Biolegend 363024), CD20-APC-Cy7 (clone 2H7, Biolegend 302314), CD27-BUV737 (clone L128, Biolegend 612829), CD38-BB700 (clone HIT2, BD 566445), IgD-BV421 (clone IA6-2, Biolegend 348226), IgG-BV786 (clone G18-145, BD 564230), IgM-PE-Cy5 (clone G20-127, BD551079) and IgA-APC (clone IS11-8E10, Miltenyi Biotech 130-113-472). .. Cells were then fixed using BD CYTOFIX for 10 minutes at room temperature followed by 90% BD Phosflow perm buffer III for 20 minutes at 4°C.

    Article Title: Bovine Lactoferrin Modulates Mononuclear Cell Activity in Human Palatine Tonsils
    Article Snippet: For intracellular marker detection (IFN-α, IFN-γ, BAFF, APRIL, and IgA), fixed cells were permeabilized with Perm/Wash Buffer (BD Biosciences) for 15 min on ice in the dark. .. The permeabilized cells were stained with IFN-α-Alexa Fluor 647, IFN-γ-PE, BAFF-PE (BD Biosciences), APRIL-APC, and IgA-APC (Miltenyi Biotec, Bergisch Gladbach, Germany) for 1 h in the dark. ..

    Article Title: Bovine Lactoferrin Modulates Mononuclear Cell Activity in Human Palatine Tonsils
    Article Snippet: For intracellular marker detection (IFN-α, IFN-γ, BAFF, APRIL, and IgA), fixed cells were permeabilized with Perm/Wash Buffer (BD Biosciences) for 15 min on ice in the dark. .. The permeabilized cells were stained with IFN-α-Alexa Fluor 647, IFN-γ-PE, BAFF-PE (BD Biosciences), APRIL-APC, and IgA-APC (Miltenyi Biotec, Bergisch Gladbach, Germany) for 1 h in the dark. ..

    Article Title: Functional insights of an uncommon hypomorphic variant in IL2RG as a monogenic cause of CVID-like disease with antibody deficiency and T CD4 lymphopenia
    Article Snippet: .. For the analysis of B cell phenotype, B cells were stained with CD27 FITC, IgG PE, CD38 APC-H7 (BD Biosciences), CD19 PeCy7 (Beckman Coulter), IgD A700 and IgM BV510 (Biolegend), IgA APC (Miltenyi), and Sytox AADvanced dead cell stain (Molecular probes). .. The analysis was performed by flow cytometry using DxFlex (Beckman Coulter) and the Flow Jo Software (LLC).

    Article Title: Functional insights of an uncommon hypomorphic variant in IL2RG as a monogenic cause of CVID-like disease with antibody deficiency and T CD4 lymphopenia.
    Article Snippet: .. For the analysis of B cell phenotype, B cells were stained with CD27 FITC, IgG PE, CD38 APC-H7 (BD Biosciences), CD19 PeCy7 (Beckman Coulter), IgD A700 and IgM BV510 (Biolegend), IgA APC (Miltenyi), and Sytox AADvanced dead cell stain (Molecular probes). .. The analysis was performed by flow cytometry using DxFlex (Beckman Coulter) and the Flow Jo Software (LLC).



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