iga apc vio770  (Miltenyi Biotec)

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 2. Total IgG, IgM and IgA antibody responses against SARS-CoV-2 antigens in ICU and non-ICU patients. (a) Frequency of positive Ig response in ICU and non-ICU patients. IgM, IgG and IgA RIs to SARS-CoV-2 antigens for (b) Envelope, (c) Nucleo, (d) Spike protein trimer and its fragments, (e) S1 and (f) RBD. A horizontal straight line represents the means, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (g) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. The significance symbols are plotted on the highest RI for each reactivity. Significance levels *: 0.01≤P≤ 0.05, **: 0.001≤P<0.01 and ***: P<0.001.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 3. Subclass of IgG and IgA antibody responses against SARS-CoV-2 antigens in ICU and non-ICU patients. (a) Frequency of positive Ig response to SARS-CoV-2 antigens. RIs to SARS-CoV-2 antigens for (b) Envelope, (c) Nucleo, (d) Spike protein trimer and its fragments, (e) S1 and (f) RBD. A horizontal straight line represents means, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (g) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. Significance levels *: 0.01≤P≤ 0.05, **: 0.001≤P<0.01 and ***: P<0.001.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 4. Total IgG, IgM and IgA antibody responses against HCoVs S1 proteins in ICU and non-ICU patients. (a) Frequency of positive Ig response. IgM, IgG and IgA RIs to HCoVs S1 proteins for (b) SARS-CoV-2, (c) SARS, (d) MERS, (e) 229E, (f) NL63, (g) HUK1 and (h) OC43. A horizontal straight line represents the mean, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (i) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. Significance levels *: 0.01≤P≤0.05, **: 0.001≤P<0.01 and ***: P<0.000.1.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test

Journal: Journal of medical microbiology

Article Title: Comprehensive analysis of human coronavirus antibody responses in ICU and non-ICU COVID-19 patients reveals IgG3 against SARS-CoV-2 spike protein as a key biomarker of disease severity.

doi: 10.1099/jmm.0.002012

Figure Lengend Snippet: Fig. 5. Subclass of IgG and IgA antibody responses against HCoVs S1 proteins in ICU and non-ICU patients. (a) Frequency of positive Ig subclass response to S1 proteins. RIs to HCoVs S1 proteins for (b) SARS-CoV-2, (c) SARS, (d) MERS, (e) 229E, (f) NL63, (g) HUK1 and (h) OC43. A horizontal straight line represents the mean, and the bars represent the sem. Unpaired two-tailed t-tests corrected for multiple comparisons by the Holm–Šidák correction were used to compare the ICU and non-ICU patients. (i) Heatmap analysis of the average of total IgG, IgM and IgA antibody responses against HCoVs S1 subunits. Yellow, green and blue indicate high, medium and low positivity index average values according to the scale of log2 of RIs. Significance levels *: 0.01≤P≤ 0.05, **: 0.001≤P<0.01 and ***: P<0.001.

Article Snippet: The microspheres in the other plate were incubated with 50 μl of assay buffer containing 1 μg ml−1 of PE- labelled mouse monoclonal antibody B3506B4 anti- IgA1 Fc (SouthernBiotech, catalogue # 9130–30) and 1 μg ml−1 of APC- labelled mouse monoclonal antibody A96042 anti- IgA2 Fc (SouthernBiotech, catalogue # 9140–31).

Techniques: Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Altered baseline immunological state and impaired immune response to SARS-CoV-2 mRNA vaccination in lung transplant recipients

doi: 10.1016/j.xcrm.2025.102050

Figure Lengend Snippet:

Article Snippet: IgA-APC , Jacksonimmuno , 109-135-011; RRID:AB_2337689.

Techniques: Virus, Recombinant, Variant Assay, Multiplexing, Amplification, RNA Sequencing, Software